A ‘Blood donation’ occurs when a person voluntarily has blood drawn and used for transfusions and/or made into biopharmaceutical medications by a process called fractionation (separation of whole-blood components). Donation may be of whole-blood (WB), or of specific components directly (the latter called apheresis). Blood banks often participate in the collection process as well as the procedures that follow it.
In the developed world, most blood donors are unpaid volunteers (voluntary non-remunerated repeat donations, VNRD) who donate blood for a community supply. In poorer countries, established supplies are limited and donors usually give blood when family or friends need a transfusion (directed donation). Many donors donate as an act of charity, but in countries that allow paid donation some donors are paid, and in some cases there are incentives other than money such as paid time off from work. Donors can also have blood drawn for their own future use (autologous donation). Donating is relatively safe, but some donors have bruising where the needle is inserted or may feel faint.
Potential donors are evaluated for anything that might make their blood unsafe to use. The screening includes testing for diseases that can be transmitted by a blood transfusion, including HIV and viral hepatitis. The donor must also answer questions about medical history and take a short physical examination to make sure the donation is not hazardous to his or her health. How often a donor can give varies from days to months based on what he or she donates and the laws of the country where the donation takes place. For example in the United States, donors must wait eight weeks (56 days) between whole blood donations but only seven days between platelet pheresis donations.[1]
The amount of blood drawn and the methods vary. The collection can be done manually or with automated equipment that only takes specific portions of the blood. Most of the components of blood used for transfusions have a short shelf life, and maintaining a constant supply is a persistent problem. This has led to some increased interest in autotransfusion, whereby a patient’s blood is salvaged during surgery for continuous reinfusion — or alternatively, is “self-donated” prior to when it will be needed. (Generally, the notion of “donation” does not refer to giving to one’s self, though in this context it has become somewhat acceptably idiomatic.)
Blood donations are divided into groups based on who will receive the collected blood.[2] An ‘allogeneic’ (also called ‘homologous’) donation is when a donor gives blood for storage at a blood bank for transfusion to an unknown recipient. A ‘directed’ donation is when a person, often a family member, donates blood for transfusion to a specific individual.[3] Directed donations are relatively rare when an established supply exists.[4] A ‘replacement donor’ donation is a hybrid of the two and is common in developing countries such as Ghana.[5] In this case, a friend or family member of the recipient donates blood to replace the stored blood used in a transfusion, ensuring a consistent supply. When a person has blood stored that will be transfused back to the donor at a later date, usually after surgery, that is called an ‘autologous’ donation.[6] Blood that is used to make medications can be made from allogeneic donations or from donations exclusively used for manufacturing.[7]
Blood is sometimes collected using similar methods for therapeutic phlebotomy, similar to the ancient practice of bloodletting, which is used to treat conditions such as hereditary hemochromatosis or polycythemia vera. This blood is sometimes treated as a blood donation, but may be immediately discarded if it cannot be used for transfusion or further manufacturing.
The actual process varies according to the laws of the country, and recommendations to donors vary according to the collecting organization.[8][9][10] The World Health Organization gives recommendations for blood donation policies,[11] but in developing countries many of these are not followed. For example, the recommended testing requires laboratory facilities, trained staff, and specialized reagents, all of which may not be available or too expensive in developing countries.[12]
An event where donors come to donate allogeneic blood is sometimes called a ‘blood drive’ or a ‘blood donor session’. These can occur at a blood bank, but they are often set up at a location in the community such as a shopping center, workplace, school, or house of worship.[13]
Donors are typically required to give consent for the process and this requirement means minors cannot donate without permission from a parent or guardian.[14] In some countries, answers are associated with the donor’s blood, but not name, to provide anonymity; in others, such as the United States, names are kept to create lists of ineligible donors.[15] If a potential donor does not meet these criteria, they are ‘deferred’. This term is used because many donors who are ineligible may be allowed to donate later. Blood banks in the United States may be required to label the blood if it is from a therapeutic donor, so some do not accept donations from donors with any blood disease.[16] Others, such as the Australian Red Cross Blood Service, accept blood from donors with hemochromatosis. It is a genetic disorder that does not affect the safety of the blood.[17]
The donor’s race or ethnic background is sometimes important since certain blood types, especially rare ones, are more common in certain ethnic groups.[18] Historically, donors were segregated or excluded on race, religion, or ethnicity, but this is no longer a standard practice.[19]
Donors are screened for health risks that could make the donation unsafe for the recipient. Some of these restrictions are controversial, such as restricting donations from MSM (men who have sex with men) for HIV risk.[20] In 2011, the UK (excluding Northern Ireland) reduced its blanket ban on MSM donors to a narrower restriction which only prevents MSM from donating blood if they have had sex with other men within the past year.[21] US senator John Kerry was pushing for the end to the 28-year-old ban in the US.[22]Autologous donors are not always screened for recipient safety problems since the donor is the only person who will receive the blood.[23] Donors are also asked about medications such as dutasteride, since they can be dangerous to a pregnant woman receiving the blood.[24]
Donors are examined for signs and symptoms of diseases that can be transmitted in a blood transfusion, such as HIV, malaria, and viral hepatitis. Screening may include questions about risk factors for various diseases, such as travel to countries at risk for malaria or variant Creutzfeldt-Jakob Disease (vCJD). These questions vary from country to country. For example, while blood centers in Québec, Poland, and many other places defer donors who lived in the United Kingdom for risk of vCJD,[25][26] donors in the United Kingdom are only restricted for vCJD risk if they have had a blood transfusion in the United Kingdom.[27]
The donor is also examined and asked specific questions about their medical history to make sure that donating blood is not hazardous to their health. The donor’s hematocrit or hemoglobin level is tested to make sure that the loss of blood will not make them anemic, and this check is the most common reason that a donor is ineligible.[28]Pulse, blood pressure, and body temperature are also evaluated. Elderly donors are sometimes also deferred on age alone because of health concerns.[29] The safety of donating blood during pregnancy has not been studied thoroughly, and pregnant women are usually deferred.[30]
The donor’s blood type must be determined if the blood will be used for transfusions. The collecting agency usually identifies whether the blood is type A, B, AB, or O and the donor’s Rh (D) type and will screen for antibodies to less common antigens. More testing, including a crossmatch, is usually done before a transfusion. Group O is often cited as the “universal donor”[31] but this only refers to red cell transfusions. For plasma transfusions the system is reversed and AB is the universal donor type.[32]
Most blood is tested for diseases, including some STDs.[33] The tests used are high-sensitivity screening tests and no actual diagnosis is made. Some of the test results are later found to be false positives using more specific testing.[34]False negatives are rare, but donors are discouraged from using blood donation for the purpose of anonymous STD screening because a false negative could mean a contaminated unit. The blood is usually discarded if these tests are positive, but there are some exceptions, such as autologous donations. The donor is generally notified of the test result.[35]
Donated blood is tested by many methods, but the core tests recommended by the World Health Organization are these four:
The WHO reported in 2006 that 56 out of 124 countries surveyed did not use these basic tests on all blood donations.[12]
A variety of other tests for transfusion transmitted infections are often used based on local requirements. Additional testing is expensive, and in some cases the tests are not implemented because of the cost.[36] These additional tests include other infectious diseases such as West Nile Virus.[37] Sometimes multiple tests are used for a single disease to cover the limitations of each test. For example, the HIV antibody test will not detect a recently infected donor, so some blood banks use a p24 antigen or HIV nucleic acid test in addition to the basic antibody test to detect infected donors during that period. Cytomegalovirus is a special case in donor testing in that many donors will test positive for it.[38] The virus is not a hazard to a healthy recipient, but it can harm infants[39] and other recipients with weak immune systems.[38]
There are two main methods of obtaining blood from a donor. The most frequent is to simply take the blood from a vein as whole blood. This blood is typically separated into parts, usually red blood cells and plasma, since most recipients need only a specific component for transfusions. A typical donation is 450 millilitres (or approximately one US pint)[40] of whole blood, though 500 millilitre donations are also common. Historically, blood donors in India would donate only 250 or 350 millilitre and donors in the People’s Republic of China would donate only 200 millilitres, though larger 300 and 400 millilitre donations have become more common.[41]
The other method is to draw blood from the donor, separate it using a centrifuge or a filter, store the desired part, and return the rest to the donor. This process is called apheresis, and it is often done with a machine specifically designed for this purpose. This process is especially common for plasma and platelets.
For direct transfusions a vein can be used but the blood may be taken from an artery instead.[42] In this case, the blood is not stored, but is pumped directly from the donor into the recipient. This was an early method for blood transfusion and is rarely used in modern practice.[43] It was phased out during World War II because of problems with logistics, and doctors returning from treating wounded soldiers set up banks for stored blood when they returned to civilian life.[44]
The blood is drawn from a large arm vein close to the skin, usually the median cubital vein on the inside of the elbow. The skin over the blood vessel is cleaned with an antiseptic such as iodine or chlorhexidine[45] to prevent skin bacteria from contaminating the collected blood[45] and also to prevent infections where the needle pierced the donor’s skin.[46]
A large[47]needle (16 to 17 gauge) is used to minimize shearing forces that may physically damage red blood cells as they flow through the needle.[48] A tourniquet is sometimes wrapped around the upper arm to increase the pressure of the blood in the arm veins and speed up the process. The donor may also be prompted to hold an object and squeeze it repeatedly to increase the blood flow through the vein.
The most common method is collecting the blood from the donor’s vein into a container. The amount of blood drawn varies from 200 milliliters to 550 milliliters depending on the country, but 450-500 milliliters is typical.[38] The blood is usually stored in a flexible plastic bag that also contains sodium citrate, phosphate, dextrose, and sometimes adenine. This combination keeps the blood from clotting and preserves it during storage.[49] Other chemicals are sometimes added during processing.
The plasma from whole blood can be used to make plasma for transfusions or it can also be processed into other medications using a process called fractionation. This was a development of the dried plasma used to treat the wounded during World War II and variants on the process are still used to make a variety of other medications.[50][51]
Apheresis is a blood donation method where the blood is passed through an apparatus that separates out one particular constituent and returns the remainder to the donor. Usually the component returned is the red blood cells, the portion of the blood that takes the longest to replace. Using this method an individual can donate plasma or platelets much more frequently than they can safely donate whole blood.[52] These can be combined, with a donor giving both plasma and platelets in the same donation.
Platelets can also be separated from whole blood, but they must be pooled from multiple donations. From three to ten units of whole blood are required for a therapeutic dose.[53] Plateletpheresis provides at least one full dose from each donation.
Plasmapheresis is frequently used to collect source plasma that is used for manufacturing into medications much like the plasma from whole blood. Plasma collected at the same time as plateletpheresis is sometimes called concurrent plasma.
Apheresis is also used to collect more red blood cells than usual in a single donation (commonly known as “double reds”) and to collect white blood cells for transfusion.[54][55]
Donors are usually kept at the donation site for 10–15 minutes after donating since most adverse reactions take place during or immediately after the donation.[56] Blood centers typically provide light refreshments or a lunch allowance to help the donor recover.[57] The needle site is covered with a bandage and the donor is directed to keep the bandage on for several hours.[40] In hot climates, donors are advised to avoid dehydration (strenuous games, alcohol) until a few hours after donation.
Donated plasma is replaced after 2–3 days.[58] Red blood cells are replaced by bone marrow into the circulatory system at a slower rate, on average 36 days in healthy adult males. In one study, the range was 20 to 59 days for recovery.[59] These replacement rates are the basis of how frequently a donor can donate blood.
Plasmapheresis and plateletpheresis donors can donate much more frequently because they do not lose significant amounts of red cells. The exact rate of how often a donor can donate differs from country to country. For example, plasmapheresis donors in the United States are allowed to donate large volumes twice a week and could nominally give 83 liters (about 22 gallons) in a year, whereas the same donor in Japan may only donate every other week and could only donate about 16 liters (about 4 gallons) in a year.[60] Red blood cells are the limiting step for whole blood donations, and the frequency of donation varies widely depending on the type of donor and local policies. For example, adult men in Hong Kong can donate once every three months, women every four months, and youth aged sixteen or seventeen only every six months.[61] In Canada and the United States it is 56 days for any type of donor.[62][63]
Donors are screened for health problems that would put them at risk for serious complications from donating. First-time donors, teenagers, and women are at a higher risk of a reaction.[64][65] One study showed that 2% of donors had an adverse reaction to donation.[66] Most of these reactions are minor. A study of 194,000 donations found only one donor with long-term complications.[67] In the United States, a blood bank is required to report any death that might possibly be linked to a blood donation. An analysis of all reports from October 2008 to September 2009 evaluated six events and found that five of the deaths were clearly unrelated to donation, and in the remaining case they found no evidence that the donation was the cause of death.[68]
Hypovolemic reactions can occur because of a rapid change in blood pressure. Fainting is generally the worst problem encountered.[69]
The process has similar risks to other forms of phlebotomy. Bruising of the arm from the needle insertion is the most common concern. One study found that less than 1% of donors had this problem.[70] A number of less common complications of blood donation are known to occur. These include arterial puncture, delayed bleeding, nerve irritation, nerve injury, tendon injury, thrombophlebitis, and allergic reactions.[71]
Donors sometimes have adverse reactions to the sodium citrate used in apheresis collection procedures to keep the blood from clotting. Since the anticoagulant is returned to the donor along with blood components that are not being collected, it can bind the calcium in the donor’s blood and cause hypocalcemia.[72] These reactions tend to cause tingling in the lips, but may cause convulsions, seizure, hypertension, or more serious problems.[73] Donors are sometimes given calcium supplements during the donation to prevent these side effects.[74]
In apheresis procedures, the red blood cells are returned. If this is done manually and the donor receives the blood from a different donor, a transfusion reaction can take place. Manual apheresis is extremely rare in the developed world because of this risk and automated procedures are as safe as whole blood donations.[75]
The final risk to blood donors is from equipment that has not been properly sterilized. In most cases, the equipment that comes in direct contact with blood is discarded after use.[76] Re-used equipment was a significant problem in China in the 1990s, and up to 250,000 blood plasma donors may have been exposed to HIV from shared equipment.[77][78]
The collected blood is usually stored in a blood bank as separate components, and some of these have short shelf lives. There are no storage solutions to keep platelets for extended periods of time, though some are being studied as of 2008.[79] The longest shelf life used for platelets is seven days.[80]
Red blood cells (RBC), the most frequently used component, have a shelf life of 35–42 days at refrigerated temperatures.[81][82] For (relatively rare) long-term storage applications, this can be extended by freezing the blood with a mixture of glycerol, but this process is expensive and requires an extremely cold freezer for storage.[38]
Plasma can be stored frozen for an extended period of time and is typically given an expiration date of one year and maintaining a supply is less of a problem.[83]
The limited storage time means that it is difficult to have a stockpile of blood to prepare for a disaster. The subject was discussed at length after the September 11 attacks in the United States, and the consensus was that collecting during a disaster was impractical and that efforts should be focused on maintaining an adequate supply at all times.[84] Blood centers in the U.S. often have difficulty maintaining even a three day supply for routine transfusion demands.[85]
The World Health Organization recognizes World Blood Donor Day on 14 June each year to promote blood donation. This is the birthday of Karl Landsteiner, the scientist who discovered the ABO blood group system.[86] The theme of the 2012 World Blood Donor Day campaign, “Every blood donor is a hero” focuses on the idea that everyone can become a hero by giving blood. As of 2008[update], the WHO estimated that more than 81 million units of blood were being collected annually.[87]
In the United States it is estimated that only 111 million citizens are eligible blood donors,[88] or 37% of the population.[89] However less than 10% donate annually.[89] In the UK the NHS reports blood donation levels at “only 4%”[90] while in Canada the rate is 3.5%.[91]
In patients prone to iron overload, blood donation prevents the accumulation of toxic quantities.[92] Donating blood may reduce the risk of heart disease for men, but the link has not been firmly established and may be from selection bias because donors are screened for health problems.[93][94]
Research published in 2012 demonstrated that repeated blood donation is effective in reducing blood pressure, blood glucose, HbA1c, low-density lipoprotein/high-density lipoprotein ratio, and heart rate.[95]
The World Health Organization set a goal in 1997 for all blood donations to come from unpaid volunteer donors, but as of 2006, only 49 of 124 countries surveyed had established this as a standard.[12] Some countries, such as Tanzania, have made great strides in moving towards this standard, with 20 percent of donors in 2005 being unpaid volunteers and 80 percent in 2007, but 68 of 124 countries surveyed by WHO had made little or no progress.[5] Most plasmapheresis donors in the United States are still paid for their donations.[96] A few countries rely on paid donors to maintain an adequate supply.[97] In some countries, for example Brazil and Australia, it is illegal to receive any compensation, monetary or otherwise, for the donation of blood or other human tissues.[98][99]
Regular donors are often given some sort of non-monetary recognition. Time off from work is a common benefit.[100] For example, in Italy, blood donors receive the donation day as a paid holiday from work.[101] Blood centers will also sometimes add incentives such as assurances that donors would have priority during shortages, free T-shirts, first aid kits, windshield scrapers, pens, and similar trinkets. There are also incentives for the people who recruit potential donors, such as prize drawings for donors and rewards for organizers of successful drives.[102] Recognition of dedicated donors is common. For example, the Singapore Red Cross Society presents awards for voluntary donors who have made a certain number of donations under the Blood Donor Recruitment Programme starting with a “bronze award” for 25 donations.[103] The government of Malaysia also offers free outpatient and hospitalization benefits for blood donors, for example, 3 months of free outpatient treatment for every donation.[104] In Poland, after donating specific amount of blood (18 liters for men and 15 for women) a person is gifted with title of “Distinguished Honorary Blood Donor” as well as a medal.
Most allogeneic blood donors donate as an act of charity and do not expect to receive any direct benefit from the donation.[105] The sociologist Richard Titmuss, in his 1970 book The Gift Relationship: From Human Blood to Social Policy, compared the merits of the commercial and non-commercial blood donation systems of the USA and the UK, coming down in favor of the latter. The book became a bestseller in the USA, resulting in legislation to regulate the private market in blood.[106] The book is still referenced in modern debates about turning blood into a commodity.[107] The book was republished in 1997 and the same ideas and principles are applied to analogous donation programs, such as organ donation and sperm donation.[108]
